il 17f ek2196 2 96 elisa kits  (Multi Sciences (Lianke) Biotech Co Ltd)

Journal: Life Science Alliance

Article Title: FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis

doi: 10.26508/lsa.202403073

Figure Lengend Snippet: (A, B) Expressions of Col2A1 (A) and Acan (B) mRNA in the entheseal tissue culture. mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (C) Production of the BMP2 protein in the entheseal tissue culture supernatant. The protein level was determined using ELISA 72 h after IL-17A stimulation. (D, E) Levels of mRNA (D) and protein (E) of FGF7 in the histoculture 72 h after IL-17A stimulation. The protein level of FGF7 in the supernatant of the entheseal tissue was measured by ELISA. (F, G) Effect of FGF7 on the expressions of Col2A1 (F) and Acan (G) mRNA in the tissue culture. The mRNA level was determined using quantitative RT–PCR 3 h after FGF7 stimulation (10 ng/ml). (H) Effect of FGF7 on the expressions of the BMP2 protein in the culture supernatant. The protein level was determined using ELISA 24 h after FGF7 stimulation (10 ng/ml). (I) Expression of IL-17RC mRNA in the histoculture. mRNA levels were determined using quantitative RT–PCR 24 h after IL-17A (10 ng/ml) or IL-17F (10 ng/ml) stimulation. The data presented are relative mRNA levels normalized to GAPDH . (A, B, C, D, E, I) Results are shown as the mean ± SD with individual data. * P < 0.05; ** P < 0.01 by Dunnett’s parametric test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice. (D, E) * P < 0.05 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). Each dot represents one experiment with pooled entheses from three to four mice.

Article Snippet: ELISA kits were used to measure IL-17A (R&D Systems), IL-17F (R&D Systems), IL-6 (R&D Systems), FGF10 (Wuhan Huamei Biotech), and FGF7 levels in the plasma.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Expressing

Journal: Life Science Alliance

Article Title: FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis

doi: 10.26508/lsa.202403073

Figure Lengend Snippet: (A) Surface expression of FGFR2IIIb on 3T3-L1 cells was analyzed by flow cytometer. The gray line indicates the histogram obtained with an isotype control. (B) Effect of the anti-FGFR2IIIb antibody on IL-13 production in FGF7-stimulated 3T3-L1 cells. The concentrations of IL-13 in the supernatant were determined by a multiplex assay. Results are shown as the % of FGF7 stimulation. IC 50 was calculated in a sigmoid dose–response curve. (C, D) Effect of the anti-FGFR2IIIb antibody on the expressions of Col2A1 (C) and Acan (D) mRNA by FGF7 stimulation. mRNA levels were determined using quantitative RT–PCR 3 h after FGF7 stimulation. (E, F) Effect of the anti-FGFR2IIIb antibody on the expressions of Col2A1 (E) and Acan (F) mRNA by IL-17A stimulation. The mRNA levels were determined using quantitative RT–PCR 72 h after IL-17A stimulation. (G) Effect of the anti-FGFR2IIIb antibody on the BMP2 production by IL-17A stimulation in the histoculture. The protein level was determined using ELISA 72 h after IL-17A stimulation. (H, I) Levels of mRNA (H) and protein (I) of FGF7. The protein level of FGF7 in the supernatant of the entheseal histoculture was measured by ELISA. The data presented are relative mRNA levels normalized to GAPDH . (C, D, E, F, G, H, I) Results are shown as the mean ± SD with individual data. * P < 0.05; ** P < 0.01 by a t test or an Aspin–Welch t test performed versus the non-stimulation group (Med). # P < 0.05; ## P < 0.01 by a t test or an Aspin–Welch t test performed versus the stimulation group (Isotype). Each dot represents one experiment with pooled entheses from three to four mice.

Article Snippet: ELISA kits were used to measure IL-17A (R&D Systems), IL-17F (R&D Systems), IL-6 (R&D Systems), FGF10 (Wuhan Huamei Biotech), and FGF7 levels in the plasma.

Techniques: Expressing, Flow Cytometry, Control, Multiplex Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Life Science Alliance

Article Title: FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis

doi: 10.26508/lsa.202403073

Figure Lengend Snippet: (A) Plasma concentrations of FGF7 were determined by ELISA at the indicated ages (wk). (B) Expression of FGF7 mRNA in both the skin and joints at the young (white column) and advanced stages (black column). The mRNA levels were determined using quantitative RT–PCR. n = 7 animals per group. (C) Gating strategy for analysis of FGFR2IIIb-expressing cells. (D, E) FGFR2IIIb expression of entheses cells of the young (D) and advanced stages (E). Gray lines indicate the histogram obtained with isotype controls. Data are representative of the stained entheseal cells of five mice. (F) Gating strategy for analysis of IL-17A–producing cells. (G, H, I, J, K, L) Intracellular IL-17A staining of skin (G, H, I) and entheseal (J, K, L) cells. (G, H, I, J, K, L) CD4 + T cells (G, J), γδ T cells (H, K), and neutrophils (I, L). (M, N, O) Number of infiltrating cells into enthesis at the young (white column) and advanced stages (black column). (M, N, O) CD4 + T cells (M), γδ T cells (N), and neutrophils (O). (A, B, M, N, O) Results are shown as the mean ± SEM with individual data. (A) ** P < 0.01 by Dunnett’s test performed versus the young stage group (6 wk). n = 5–11 animals per group. (B) ** P < 0.01 by an Aspin–Welch test performed versus the young stage group. n = 7 animals per group. (M, N, O) * P < 0.05 by an Aspin–Welch test performed versus the young stage group. n = 3–5 animals per group.

Article Snippet: ELISA kits were used to measure IL-17A (R&D Systems), IL-17F (R&D Systems), IL-6 (R&D Systems), FGF10 (Wuhan Huamei Biotech), and FGF7 levels in the plasma.

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Staining

Journal: Life Science Alliance

Article Title: FGF7 as an essential mediator for the onset of ankylosing enthesitis related to psoriatic dermatitis

doi: 10.26508/lsa.202403073

Figure Lengend Snippet: (A, B, C, D) ELISA kits were used to measure IL-17A (A), IL-17F (B), IL-6 (C), and FGF7 (D) in the plasma at the experimental endpoint. (E, F, G, I) Quantitative RT–PCR was used to measure IL-17A (E), IL-17F (F), IL-6 (G), FGF7 (H), and IL-17RC (I) in the ankle joint at the experimental endpoint. (J, K, L, M, N, O) Quantitative RT–PCR was used to measure IL-17A (J), IL-17F (K), IL-6 (L), Keratin 16 (M), S100A8 (N), and S100A9 (O) in the skin of the tail base at the experimental endpoint. Results are shown as the mean ± SEM with individual data. * P < 0.05; ** P < 0.01 by a t test or an Aspin–Welch t test performed versus the young group (6 wk). # P < 0.05; ## P < 0.01 by a t test or an Aspin–Welch t test performed versus the isotype control antibody (Isotype). n = 8 animals per group.

Article Snippet: ELISA kits were used to measure IL-17A (R&D Systems), IL-17F (R&D Systems), IL-6 (R&D Systems), FGF10 (Wuhan Huamei Biotech), and FGF7 levels in the plasma.

Techniques: Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Quantitative RT-PCR, Control